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Class 12 Biology Objective In English Chapter – 11 Biotechnology: Principles and Processes
1. For induction of alien DNA is host cell we may use-
(A) Gene gun
(B) Micro-pipette
(C) Both (A) and (B)
(D) None of these
Ans-( C )
2. (amp)R gene is responsible for developing resistance in
(A) Pest
(B) Insect
(C) Antibiotic
(D) Spherosome
Ans-( C )
3. Amplification of gene for interest may be done by
(A)MMR
(B) PCR
(C) MRI
(D) All of these
Ans-( B )
4. Bio reactors provide optimal conditions to produce desired-
(A) Product
(B) Organism
(C) Medium
(D) All of these
Ans-( A )
5. Restriction enzymes are known as
(A) Biological guns
(B) Molecular scissors
(C)Plasmid
(D) Micro Pipette
Ans-( B )
6. The most common bacterium used in genetic engineering is :
(A) Escherichia
(B) Clostridium
(C) Salmonella
(D) Bacillus
Ans-( A )
7. Genetic engineering is also called
(A)Recombinant DNA technique
(B) Tissue culture
(C) Cloning vector
(D ) Flow of resources
Ans-( A )
8. Which in the following is not Restriction enzyme ?
(A) EcoR1
(B) Bam H1
(C) Hind III
(D) Pectinase
Ans-( D )
9. Which one is used to observe DNA?
(A) Ethidium bromide
(B) Aniline blue
(C) Saffarine
(D) Fast green
Ans-( A )
10. Bam H1 restriction enzyme is obtained from
(A) Haemophilus influenzae
(B) E. Coli
(C) Bacillus emaloliquifaciens
(D) None
Ans-( C )
11. DNA polymerase can be obtained from-
(A) PBR 322
(B) Thermus aquaticus
(C) Agrobacterium
(D) Retrovirus
Ans-( B )
12. In Eco RI of Restriction endonuclease Co represents
(A) Colon
(B) Coelom
(C) Co-enzyme
(D) Coli
Ans-( D )
13. Fungus is filtered by which enzyme?
(A) Chitinase
(B) Lysozyme
(C) Cellulose
(D) Galactose
Ans-( A )
14. “Restriction’ enzyme is used to splices which of the following ?
(A) SS-RNA
(B) ds-DNA
(C)SS-DNA
(D) ds-RNA
Ans-( B )
15. Enzyme ligase is used in
(A) Splitting of DNA
(B) Ligation of broken DNA
(C)To purify DNA
(D) To enhance production efficiency of Bioreactors.
Ans-( B )
16. Ti-plasmid is found in :
(A) Agrobacterium tumifaciens
(B) E.Coli
(C) B.coli
(D) All of these
Ans-( A )
17. Agarose is extracted from
(A) See weeds
(B) Maize
(C) Cycas
(D) None of these
Ans-( A )
18. What is true of plasmids ?
(A) They are found in viruses
(B) They are main parts of chromosomes
(C) They are widely used in gene transfer
(D) They contain genes for vital activities
Ans-( C )
19. ‘Cry gene’ prevents which crop from bolloworms ?
(A) Cotton
(B) Mango
(C) Tea
(D) Wheat
Ans-( A )
20. Bacteria has
(A) Plasmid DNA
(B) RNA
(C) Both (A) and (B)
(D) None of these
Ans-( C )
21. A molecular technique in which multiple copy of the desired gene is synthesized in vitro is called as
(A) ELISA
(B) PCR
(C) Gel Electrophoresis
(D) Flow cytometry
Ans-( B )
22. Probiotics are
(A) A new kind of food allergen
(B)Safe antibiotics
(C) Live microbial food supplement
(D)Cancer inducing microbes
Ans-( C )
23. Which of the following techniques can be to introduce foreign DNA into cell ?
(A) Using disarmed pathogen
(B) Microinjection
(C) Gene gun
(D)All of above
Ans-( D )
24. T-DNA is found in :
(A)Ti-plasmid
(B) phagemid
(C) PBR 322
(D) PUC 18
Ans-( A )
25. The first restriction enzyme used in genetic engineering is :
(A) Eco RI
(B) Hind III
(C) Eco RV
(D) None of above
Ans-( A )
26. The restriction site chain present inE. Coli.
(A) Eco RI
(B) Hind III
(C) Bam HI
(D) All of above
Ans-( D )
27. Which vector is used in cloning of plants ?
(A) PBR 322
(B) Ti Plasmid
(C)Both (A) and (B)
(D) None
Ans-( C )
28. Which of the following sequences are recognised by EcoRI restriction enzyme ?
(A) GAATTC
(B) GGCC
(C) AGGCCT
(D) TCCGGA
Ans-( A )
29. Which cry gene in the following prevents boring of crop?
(A) cryIAC
(B) cryIIAb
(C)crylab
(D) All of above
Ans-( D )
30. “Molecular scissors” used in genetic engineering is :
(A)DNA ligase
(B) DNA polymerase
(C) Helicase
(D)Restriction endonuclease
Ans-( D )
31. Which is used extensively for genetic engineering in plants ?
(A) Agrobacterium tumefaciens
(B) Xanthomonas citri
(C) Bacillus coagulens
(D) Clostridium septicum
Ans-( A )
32.PCR is required for :
(A) DNA synthesis
(B) DNA amplification
(C) Protein synthesis
(D ) Amino acid synthesis
Ans-( B )
33. Restriction endonuclease is most widely used in DNA recombinant Technology. It is obtained from :
(A) bacteriophage
(B) bacterial cells
(C) plasmids
(D) all prokaryotic cells
Ans-( B )
34. Bacteria protects themselves from viral attack by producing :
(A) Exonuclease
(B) Endonuclease
(C) DNA Ligage
(D) Gyrase
Ans-( B )
