1. A molecular technique in which multiple copy of the desired gene is synthesized in vitro is called as
(A) ELISA
(B) PCR
(C) Gel Electrophoresis
(D) Flow cytometry
Answer-( B )
2. Probiotics are
(A) A new kind of food allergen
(B)Safe antibiotics
(C) Live microbial food supplement
(D)Cancer inducing microbes
Answer-( C )
3. Which of the following techniques can be to introduce foreign DNA into cell ?
(A) Using disarmed pathogen
(B) Microinjection
(C) Gene gun
(D)All of above
Answer-( D )
4. T-DNA is found in :
(A)Ti-plasmid
(B) phagemid
(C) PBR 322
(D) PUC 18
Answer-( A )
5. The first restriction enzyme used in genetic engineering is :
(A) Eco RI
(B) Hind III
(C) Eco RV
(D) None of above
Answer-( A )
6. The restriction site chain present inE. Coli.
(A) Eco RI
(B) Hind III
(C) Bam HI
(D) All of above
Answer-( D )
7. Which vector is used in cloning of plants ?
(A) PBR 322
(B) Ti Plasmid
(C)Both (A) and (B)
(D) None
Answer-( C )
8. Which of the following sequences are recognised by EcoRI restriction enzyme ?
(A) GAATTC
(B) GGCC
(C) AGGCCT
(D) TCCGGA
Answer-( A )
9. Which cry gene in the following prevents boring of crop?
(A) cryIAC
(B) cryIIAb
(C)crylab
(D) All of above
Answer-( D )
10. “Molecular scissors” used in genetic engineering is :
(A)DNA ligase
(B) DNA polymerase
(C) Helicase
(D)Restriction endonuclease
Answer-( D )
11. Which is used extensively for genetic engineering in plants ?
(A) Agrobacterium tumefaciens
(B) Xanthomonas citri
(C) Bacillus coagulens
(D) Clostridium septicum
Answer-( A )
12. PCR is required for :
(A) DNA synthesis
(B) DNA amplification
(C) Protein synthesis
(D ) Amino acid synthesis
Answer-( B )
13. Restriction endonuclease is most widely used in DNA recombinant Technology. It is obtained from :
(A) bacteriophage
(B) bacterial cells
(C) plasmids
(D) all prokaryotic cells
Answer-( B )
14. Bacteria protects themselves from viral attack by producing :
(A) Exonuclease
(B) Endonuclease
(C) DNA Ligage
(D) Gyrase
Answer-( B )
15. For induction of alien DNA is host cell we may use-
(A) Gene gun
(B) Micro-pipette
(C) Both (A) and (B)
(D) None of these
Answer-( C )
16. (amp)R gene is responsible for developing resistance in
(A) Pest
(B) Insect
(C) Antibiotic
(D) Spherosome
Answer-( C )
17. Amplification of gene for interest may be done by
(A)MMR
(B) PCR
(C) MRI
(D) All of these
Answer-( B )
18. Bio reactors provide optimal conditions to produce desired-
(A) Product
(B) Organism
(C) Medium
(D) All of these
Answer-( A )
19. Restriction enzymes are known as
(A) Biological guns
(B) Molecular scissors
(C)Plasmid
(D) Micro Pipette
Answer-( B )
20. The most common bacterium used in genetic engineering is :
(A) Escherichia
(B) Clostridium
(C) Salmonella
(D) Bacillus
Answer-( A )
